Development of a competitive fluorescence-based synaptosome binding assay for brevetoxins
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چکیده
منابع مشابه
A New Cytotoxicity Assay for Brevetoxins Using Fluorescence Microscopy
Brevetoxins are a family of ladder-framed polyether toxins produced during blooms of the marine dinoflagellate, Karenia brevis. Consumption of shellfish or finfish exposed to brevetoxins can lead to the development of neurotoxic shellfish poisoning. The toxic effects of brevetoxins are believed to be due to the activation of voltage-sensitive sodium channels in cell membranes. The traditional c...
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1. A competitive protein-binding assay for plasma 25-hydroxycholecalciferol was developed, using Sephadex LH-20 columns for the isolation of the sterol and a partially purified protein from rat serum as the binding protein. 2. The mean plasma 25-hydroxycholecalciferol in eighteen male and female normal volunteers was 38 5 SD 14 pmol/ml (1 5.2 & 5.6 nglml). 3. Levels of 25-hydroxycholecalciferol...
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A solid-phase enzyme-receptor assay (sPcR.A) has been developed for glycopeptide antibiotics of the vancomycin class such as teicoplanin, vancomycin, ristocetin, avoparcin, actaplanin,A-47934, A-41 030, and A-3551 2-B. The assay exploitsthe mechanismof most action of these antibiotics, which is based on their interaction with acyl-D-alanyl-Dalanine, a constituentof the walls of mostgrowingbacte...
متن کاملDevelopment of a Fluorescence Assay for the Characterization of Brevenal Binding to Rat Brain Synaptosomes
The marine dinoflagellate Karenia brevis produces a family of neurotoxins known as brevetoxins. Brevetoxins elicit their effects by binding to and activating voltage-sensitive sodium channels (VSSCs) in cell membranes. K. brevis also produces brevenal, a brevetoxin antagonist, which is able to inhibit and/or negate many of the detrimental effects of brevetoxins. Brevenal binding to VSSCs has ye...
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ژورنال
عنوان ژورنال: Harmful Algae
سال: 2012
ISSN: 1568-9883
DOI: 10.1016/j.hal.2012.06.003